Effect of two Exercise Training Protocols on miR-۱۳۳a and Runx۲ in High-Fat Diet and Streptozotocin-induced Diabetes
Publish place: Research in Molecular Medicine، Vol: 9، Issue: 3
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_REMJ-9-3_002
تاریخ نمایه سازی: 20 دی 1402
Abstract:
Background: Type ۲ Diabetes (T۲D) in the elderly is an epidemic that significantly impacts global health. This experimental study aimed to compare the responses of microRNA-۱۳۳a (miR-۱۳۳a) in different tissues and of Runt-related transcription factor ۲ (Runx۲) in bone marrow tissue following resistance and endurance training in old rats with High-Fat Diet and Streptozotocin (HFD/STZ)- induced type ۲ diabetes.
Materials and Methods: T۲D was induced by HFD/low-dose STZ in ۳۰ male Wistar rats (۲۱-monthold, Mean±SD weight ۴۱۸±۴۳ g). The rats received HFD (۵۵%, ۳۱%, and ۱۴% of energy from fat, carbohydrate, and protein, respectively; ۵.۲ kcal/g). The diets continued for eight weeks in both groups. Over week four, the rats in the group with HFD/STZ-induced T۲D received treatment with low-dose STZ. After one week of familiarity with the laboratory environment, they were randomly divided into three groups: Diabetic Endurance Training (DET, n=۱۰), Diabetic Resistance Training (DRT, n=۱۰), and Diabetic Control (DC, n=۱۰). The eight weeks of endurance training protocol comprised five sessions of moderate-intensity training (۶۰%-۷۵% velocity at maximal oxygen uptake (vVO۲max) and low intensity (۳۰%-۳۰% vVO۲max). In ۶۰% Maximum Voluntary Carrying Capacity (MVCC), the resistance group climbed the ladder ۱۴-۲۰ times with ۱-minute rest, five days a week.
Results: The results of the ۱-way ANOVA test showed no significant change in serum miR-۱۳۳ expression (P=۰.۴۱۱) and muscle tissue (P=۰.۰۷۷) following resistance and endurance training. However, significant differences were observed in bone marrow miR-۱۳۳ expression (P=۰.۰۰۳) and Runx۲ gene expression (P=۰.۰۰۲) between groups. Tukey’s post hoc tests showed that the bone marrow miR-۱۳۳ expression had a significant increase following eight weeks of resistance training compared to the endurance training (P=۰.۰۰۶) and control (P=۰.۰۰۲) groups, and bone marrow Runx۲ gene expression in rats exposed to resistance training compared to the endurance training (P=۰.۰۴۴) and the control (P=۰.۰۱۸) groups.
Conclusion: It seems that longer periods of exercise are required for cellular changes in the metabolism of these tissues after these exercise protocols. This topic should be studied in future research.
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Authors
Maryam Asadi Farsani
Shahrekord university
Ebrahim Banitalebi
Shahrekord university
Mohammad Faramarzi
Esfahan University, Esfahan, Iran.
Nuredin Bakhtiari
Department of Biochemistry, Faculty of Biological Sciences, North- Branch of Tehran, Islamic Azad University, Tehran, Iran
Mostafa Rahimi
Shahrekord university
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