Association of Toxoplasma Gondii Infection With Diabetes Mellitus Using Nested-PCR and Sequencing
Publish place: Research in Molecular Medicine، Vol: 9، Issue: 2
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
View: 51
This Paper With 8 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_REMJ-9-2_002
تاریخ نمایه سازی: 20 دی 1402
Abstract:
Background: Toxoplasmosis is a global public health concern with severe complications, particularly in pregnant women and immunosuppressed patients. Herein, we revealed the first genotypic evidence of Toxoplasma gondii (T. gondii) in diabetic and non-diabetic individuals referred to Imam Sajjad Hospital, Tehran, Iran, using nested-Polymerase Chain Reaction (PCR) and sequencing.
Materials and Methods: We collected ۹۸, ۹۵, and ۹۴ blood samples from Type-۱ Diabetes Mellitus (T۱DM), Type-۲ Diabetes Mellitus (T۲DM), and control subjects from February to August ۲۰۱۸, and their DNA was extracted. After amplification and visualization of dense granule antigen ۶ gene (۳۴۴ bp) using external and internal primer pairs, the positive samples were sent for sequencing.
Results: Among ۲۸۷ individuals, three T۱DM patients (two IgG, one IgM) and one T۲DM patient (IgG) were found positive by molecular method, which proved to be type III strain by sequencing.
Conclusion: To the best of our knowledge, this is the first genotyping investigation on T. gondii in diabetic individuals. Further research should be done to better realize the association between Toxoplasma genotypes and the outcome of diabetes mellitus.
Keywords:
Authors
Sadegh Shamsinia
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Abdolhossein Dalimi
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Majid Pirestani
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Hamidreza Majidiani
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :