Histopathologic Evaluation of Radio-Protective Effect of Hesperidin on the Liver of Sprague Dawely Rats

Background: Hesperidin is a bioflavonoid glycoside mainly found in citrus fruit and has been shown radio-protective potential in various measurement systems. Objective: In this article aims to investigate the radio-protective effect of hesperidin on the liver of Sprague Dawely rats. Material and Methods: In this clinical study, ۴۰ male rats were selected randomly and divided into ۸ groups. Group ۱ did not receive radiation and hesperidin (sham control). Group ۲ received only ۱۰۰ mg/kg body weight (b.w) of hesperidin for ۷ consecutive days (HES group); group ۳ exposed to dose of ۲Gy whole body gamma radiation (۲Gy group), and group ۴ and ۵ received ۵۰ and ۱۰۰ mg/kg b.w of HES for ۷ consecutive days before ۲ Gy gamma radiation, respectively.Group ۶ exposed to dose of ۸Gy gamma radiation (۸Gy group); group ۷ and ۸ received ۵۰ and ۱۰۰ mg/kg b.w of HES for ۷ days before ۸Gy gamma irradiation, respectively. Histopathological evaluation was perfomred ۲۴ hours after radiation. Results: Administration of hesperidin (۵۰ mg/kg b.w, ۷ days) before ۲Gy of gamma irradiation led to remove inflammatory mononuclear cells in the portal space. Microscopic findings in the groups receiving two doses of hesperidin (۵۰ and ۱۰۰ mg/kg b.w, orally, ۷ days), before ۸Gy of gamma radiation, were similar in a way that extreme dilation of central veins to be seen, however, there was no capillarization. Conclusion: HES can be offered as a suitable radio-protector in radiotherapy patients and radiation workers.

Keywords:

Gamma rays , Hesperidin , Radio-protector , Hyperemia , Capillarization