The Seed Priming using Putrescine Improves, Germination Indices and Seedlings morphobiochemical Responses of Indigo (Indigofera tinctoria) under Salinity Stress

This research was done as a factorial experiment with ۵ replications was carried out. The seeds were primed using putrescine at concentrations of ۰ (hydropriming), ۰.۵, and ۱ mM, and control were used as control. Salinity treatment was applied under normal, low, moderate and severe (۰, ۵۰, ۱۰۰, and ۱۵۰ mM NaCl, respectively) salinity stress. According to the findings, increasing salinity levels reduced germination percentage, (GP), germination rate, (GR), coefficient of velocity of germination, (CVG), seed weight vigour index, (SWVI), by ۲۹, ۶۶, ۵۳ and ۲۵%, respectively and seedling fresh weight, seedling dry weight, roots length and shoot length by ۳۷, ۱۴, ۷۲ and ۶۱%, respectively, while increasing mean germination time (MGT), percentage of dry matter, total soluble sugar (TSS), reactive oxygen species (ROS) and malondialdehyde (MDA) by ۱۱۲, ۵۳, ۵۷,۱۶ and ۱۸۲%, respectively. When seeds were primed using putrescine reduced the effects of oxidative stress by reducing ROS and MDA levels by ۱۱ and ۴۷%, respectively compared to control and improved the GP, GR, CVG, SWVI, root length and shoot length by ۳۱, ۸۱, ۶۷, ۳۶, ۲۷, ۱۹%, and decreased MGT by ۴۰%. Although seed priming using ۱ mM putrescine was effective in some parameters, there was no significant difference when compared to ۰.۵ mM putrescine. As a result, seed priming indigo using ۰.۵ mM putrescine with increasing dry matter by ۷۳% appears to be more practical and economical in terms of mitigating the negative effects of salinity stress.

Keywords:

dry matter percentage , germination percentage , Indigo plant , Reactive oxygen species