Acute and Subchronic Toxicity and Cytotoxicity of Saffron Corm Extract

Publish Year: 1403
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JJMPB-13-1_002

تاریخ نمایه سازی: 8 بهمن 1402

Abstract:

Saffron corm is produced annually in large quantities in Iran. It contains some bioactive components like phenolic and antioxidative compounds. We extracted saffron corm by ultrasound-assisted solvent extraction method. The extraction yield and antioxidant properties, free radical scavenging ability, FRAP, and total phenolic compounds (TPC), were determined. To evaluate acute and subchronic toxicity, the extract was administered at three different doses of ۱, ۱۰, and ۱۰۰ mg/kg to BALB/c male mice. Then, the rate of mortality and biochemical parameters such as LDH, ALT, AST, ALP, TG, cholesterol and creatinine were measured after ۲۴ hours and ۶۰ days respectively. Effect of extract on healthy (HFF) and cancerous cells (MCF-۷ & HT-۲۹) was evaluated using MTT assay. Results showed that the ۴۰ min ultrasound-assisted extraction method with the highest extraction yield (۰.۹۸%), free radical scavenging inhibition (۶۴.۳۷%), and highest FRAP (۶۴۳.۵۱ µmolfeII.l-۱) and TPC (۸۲.۲۳ mg.ml-۱) could be selected as the most active saffron corm extract. According to our animal study after acute and subchronic administration of extract, no significant alteration of the levels of TG, cholesterol, ALP, ALT, AST and creatinine was observed, in mice. Cytotoxicity tests showed that saffron corm extract had no toxic effect on HFF cells, although it had toxic effects on MCF-۷ & HT-۲۹ cells. Conclusively, to our data, saffron corm extract had no significant acute and subchronic toxicity on BALB/c mice, and although it had toxic effects on cancer cells no cytotoxicity was seen on normal fibroblast cells.

Authors

Soodabeh Einafshar

Agricultural Engineering Research Department, Khorasan Razavi Agricultural and Natural Resources and Education Center, AREEO, Mashhad, Iran

Fatemeh Tavakoli

Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Zahra Hakimi

Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Azadeh Emami

Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

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