The effect of high- intensity interval training with silymarin extract on malondialdehyde and superoxide dismutase in heart and lung endothelial tissue of male rats exposed to diazinon

Introduction and aim: Diazinon is an organophosphorus insecticide that causes oxidative stress. However, physical activity and consumption of antioxidants improve the antioxidant status of the body. The aim of the present study was to investigate the effect of high- intensity interval training with silymarin extract on MDA and SOD indices of heart and lung endothelial tissues in male rats exposed to diazinon. Methods: In this experimental study, 36 male Sprague-Dawley rats with a weight range of 150 to 200 grams and an average age of 8 weeks were randomly assigned to 1) control (C), 2) sham (Sh) 3) diazinon. (D) 4) silymarin extract (SL), 5) high- intensity interval training (TH) and 6) high- intensity interval training with silymarin extract (TH+SL) were divided. During 8 weeks, rats in groups 3 to 6 received diazinon 1.5 mg/kg intraperitoneally five days a week. Groups 5 and 6 performed HIIT exercises five times a week, and groups 4 and 6 received 50 mg/kg silymarin extract intraperitoneally five days a week. high- intensity interval training with an intensity of 85% to 110% VO2max and a speed of 15-45 m/min was performed for 8 weeks. After 8 weeks, malondialdehyde (MDA) levels and superoxide dismutase (SOD) gene expression were measured in heart and lung endothelial tissues. To analyze the findings, Shapiro-Wilk test, one-way analysis of variance along with Tukey's post hoc test were used in SPSS version 22 software. Also, (P≤0.05) was considered as a significant level. Findings: Diazinon had a significant effect on the increase of MDA and decrease of SOD in heart and lung endothelial tissues (P<0.05). However, HIIT exercise and consumption of silymarin each alone lead to a decrease in MDA levels and an increase in SOD in heart tissues. and lung endothelial of the poisoned mice (P<0.05). Meanwhile, both HIIT exercise interventions with silymarin had a greater effect on reducing MDA and increasing SOD in heart and lung endothelial tissues of rats exposed to diazinon (P<0.05). Conclusion: It seems that regular HIIT exercises and consumption of silymarin each alone and the interaction of the two together can cause a decrease in oxidative indices and an increase in antioxidants in heart and lung endothelial tissues caused by diazinon poisoning.

The effect of high- intensity interval training with silymarin extract on malondialdehyde and superoxide dismutase in heart and lung endothelial tissue of male rats exposed to diazinon Keywords:

Malondialdehyde , Superoxide dismutase , high- intensity interval training , silymarin , diazinon