Distribution of Estrogen Receptor ۱ (ESR۱) gene (rs۲۲۳۴۶۹۳) polymorphism among breastcancer patients from Mazandaran province, Iran; a case-control study
Publish Year: 1402
نوع سند: مقاله کنفرانسی
زبان: English
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ZISTCONF02_014
تاریخ نمایه سازی: 16 بهمن 1402
Abstract:
Background: Breast cancer accounts for about one-third of woman’s cancers today and theincidence of breast cancer has been increasing in Iranian women, recently. Changes in the estrogen signaling pathway, including changes in the estrogen receptor α (ESR۱) gene, play an important role in the development of breast cancer, especially in the whites. The current study was conducted to investigate the distribution of the rs۲۲۳۴۶۹۳ ESR۱ polymorphism in breast cancer patients.Methods: Peripheral blood samples were taken from ۶۰ breast cancer patients and ۶۰ healthy individuals, referring to the Imam Khomeini hospital, Sari, Iran, to determine the (rs۲۲۳۴۶۹۳ T>C) single nucleotide polymorphism (SNP). Genomic DNA was extracted using the salting-out method and genotyping was performed by PCR-RFLP assay. Statistical analysis was performed using Medcalc version ۱۵ software. Results: frequency of TC, TT, and CC genotypes among healthy controls were ۶۵%, ۱۵%, and ۲۰%, respectively. The patient group had a frequency of ۳۸.۵%, ۳۸.۵%, and ۲۳% for TC, TT, and CC genotypes, respectively. Our results showed a significant difference in the prevalence of ESR۱ gene genotypes between patients and control groups (χ۲= ۱۰.۴۰۸, p= ۰.۰۰۵).Conclusion: This study showed that the ESR۱ polymorphism may be involved with the increased risk of breast cancer development and the rs۲۲۳۴۶۹۳ T>C SNP could be regarded as potent marker for breast cancer screening approaches.
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Authors
Pardis Mirzaeyan
Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran;
Mohammad Shokrzadeh
Pharmacutical Research Center, Department of Toxicology and Pharmacology, Faculty ofPharmacy, Mazandaran University of Medical Sciences, Sari, Iran;
Ali Salehzadeh
Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran;
Farzam Ajamian
Department of Biology, Faculty of science, University of Guilan, Rasht, Iran;