Increased levels of serum Adenosine deaminase (ADA) enzyme and increased risk of T cell activation markers in type ۲ diabetes

Diabetes Type ۲ is the most common type of diabetes, a common disorder of glucose homeostasis and accounts for ۹۰% of cases. The prevalence of diabetes type ۲ is increasing. Adenosine deaminase is an enzymatic polymorphism that plays an important role in modulating the biological activity of insulin. It seems that excessive activity of the adenosine A۱ receptor has caused adiposity in diabetes type ۲. In this study, we examined the correlation of ADA enzyme with diabetes type ۲. This investigation was performed on ۸۰ men and women between ۴۰ and ۸۰ years old in District ۲ of Tehran with diabetes. Venous blood samples were collected after ۱۲ hours of fasting blood was centrifuged. Then fasting blood glucose and HbA۱c, Triglyceride, and total Cholesterol were measured for enzyme activity respectively by COBAS MIRA. Insulin was measured by ELISA and serum ADA enzyme activity was measured by photometry. The results of this study were done by SPSS software. A significant increase in serum ADA levels was observed in diabetic patients compared with the control group. A positive correlation was observed between ADA activity and FBS and HbA۱c. The amount of HOMA-IR in diabetics was higher than in the control group, but no positive correlation was observed between serum levels of ADA and HOMA-IR. The enzyme adenosine deaminase can act as an immunological marker and the results of this study show that diabetes is associated with increased T cell activation markers and immune disequilibrium. Serum ADA level has a positive correlation with glycemic control status in patients.

Keywords:

Adenosine deaminase , Diabetes , Glycosylted hemoglobin , HbA۱c , Hyperlipidemia , Hypertension