Antioxidant Properties of Resveratrol on Acetaminophen Induced Toxicity in Wistar Rat Liver and HepG۲ Cells

Background: Although acetaminophen (APAP) is considered safe at therapeutic doses, intake of high amounts of this drug can cause liver failure. In the present experiment, we examined the hepatoprotective effects of resveratrol (RES) in HepG۲ cells and rat liver. Objectives: This study aimed to evaluate the influence of RES on liver function in rat model of necrosis and HepG۲ cells. Materials and Methods: In this study, rats were randomly assigned into ۴ groups (۷ rats in each group) as follows; group ۱: control rats (received normal saline), group ۲: hepatotoxic control (control rats that received ۶۴۰ mg/kg/d APAP), group ۳: positive control (received ۱۵۰ mg/kg N-acetylcysteine), group ۴: RES (received ۳۰ mg/kg RES). The animals were treated for ۷ days. Afterwards, the levels of liver enzymes, protein carbonyl content, glutathione (GSH) level, and Tumor necrosis factor (TNF-α) level were determined. Results: In the in vitro experiment, APAP-induced HepG۲ cells were treated with RES at different concentrations and various factors such as cell viability, liver enzymes, GSH and TNF-α levels were measured. Conclusions: Our results indicated that RES could normalize all these factors in vitro and in vivo (P<۰.۰۵). In fact, RES had potential hepatoprotective effect against APAP -induced hepatotoxicity in HepG۲ cells and animal models mainly via dual change of oxidative stress and cytokine levels.