Beneficial Effects of Renin-Angiotensin System Blockers on Testicular Morphology

Background: The renin-angiotensin system (RAS) is a set of peptides, enzymes, and receptors specially involved in the control of extracellular fluid volume and blood pressure (BP); however, some of its components have already been identified in the testis, such as angiotensinogen, angiotensin converting enzyme, and renin. Objectives: The aim of this study was to evaluate whether renin-angiotensin system blockers have effects on the testicular morphology of animals fed a high energy density (HED) diet. Materials and Methods: Male C۵۷BL/۶ mice were fed initially a standard chow (SC) or a HED diet. After ۸ weeks, HED animals were randomized into ۴ groups, each group receiving one of the following treatments for the next ۶ weeks: HED-A: aliskiren (۵۰ mg/kg/d); HED-E: enalapril (۳۰ mg/kg/d); HED-L: losartan (۱۰ mg/kg/d); and untreated HED group. The BP was measured biweekly. At the end of treatment, all animals were killed and the testes were processed for morphometric and stereological parameters including density of seminiferous tubules per area, density of length and the total length of the seminiferous tubules, height of the epithelium, and diameter of the seminiferous tubules. Samples were tested for their homoscedasticity and the differences between the groups were tested by one-way analysis of variance (ANOVA), followed by the Holm-Sidak post-test. In all cases, the significance level adopted was P ≤ .۰۵. Results: Compared to SC, HED groups presented an increase in BP, normalized by all RAS blockers. However, the HED diet caused testis alterations that were not affected by aliskiren or losartan. Only enalapril maleate was capable of reversing such alterations. Conclusions: Further studies are still needed to answer why only enalapril was able to reveal the morphological changes caused by the high energy diet; so enalapril could be suggested as the drug of choice for patients with previous reproductive dysfunction.