Genotypic Investigation of Antibiotic Resistant blaOXA-۴ Gene in Clinical Isolates of Pseudomonas aeruginosa

Background: Pseudomonas aeruginosa, an opportunistic Gram-negative bacterium, is responsible for ۱۰-۱۵% of hospital infections worldwide. The acquisition of resistance genes is one of the important mechanisms that causes the spread of resistance in this bacterium. This study aimed to conduct a phenotypic and genotypic investigation of the blaOXA-۴ resistance gene in P. aeruginosa isolated from clinical samples. Methods: In this study, ۱۱۰ P. aeruginosa strains were isolated from various clinical samples. The disk diffusion method was applied to reveal the resistance pattern in the isolates. Moreover, the combined disk method was used for the phenotypic analysis of extended-spectrum beta-lactamases (ESBL). Finally, the presence of the blaOXA-۴ beta-lactamase gene was analyzed genotypically by polymerase chain reactions (PCR) method. Results: The highest sensitivity and resistance of the isolates were related to amikacin (۶۵.۴۵%) and ceftazidime (۸۶.۳۶%), respectively. The phenotypic analysis indicated that ۷۲ isolates (۶۵.۴۵%) of P. aeruginosa are ESBL-producing. Furthermore, the presence of blaOXA-۴ was approved genotypically in ۳۳ P. aeruginosa isolates (۴۵.۸۳%). Conclusion: This study revealed a high prevalence of antibiotic-resistant isolates of P. aeruginosa in the East Azerbaijan population that may be associated with the presence of the blaOXA-۴ gene. However, further studies are necessary to identify other resistant genes in ESBL-producing isolates and other geographical areas with larger sample size.