Phenotypic and Genotypic Characteristics of Listeria monocytogenes Isolated From Dairy and Meat Products
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JCMI-2-3_003
تاریخ نمایه سازی: 27 بهمن 1402
Abstract:
Background: Listeria monocytogenes is a foodborne pathogen and a serious threat to the public health in the world. Consumption of traditional foods such as dairy and meat products can be a major reason for relative abundance and isolation of these bacteria. Objectives: The purpose of this study was to determine the phenotypic and genotypic characteristics of L. monocytogenes strains isolated from dairy and meat products. Materials and Methods: A total of ۳۱۷ dairy products and meat-processed samples were collected. Antibiotic susceptibility test was performed on each sample by the disk diffusion method (Kirby Bauer). Five reference loci were used for typing of L. monocytogenes strains by MLVA (Multiple Locus VNTR Analysis) Technique. Results: A total of ۲۴ L. monocytogenes isolates were collected from the dairy and meat products. Resistance of isolated L. monocytogenes strains to penicillin G were ۵۴.۵۴% (from dairy products) and ۴۶.۱۵% (from processed meat). Genetic relatedness of isolates were assessed by MLVA. Out of ۱۳ different types, type ۲ with ۶ strains and type ۳ with ۴ strains, were the most common types. Conclusions: MLVA analysis showed that samples obtained from different sources could have similar genetic profile. As a result, administration of penicillin in patients with listeriosis (especially pregnant women) and antibiotic susceptibility test are recommended. The fast and accurate methods such as MLVA for tracking of pollution sources of L. monocytogenes are recommended during outbreaks.