The combined effect of eight-week resveratrol supplementation and interval training on some regulatory and executive factors of hepatocyte apoptosis in male rats with diabetes

Background and aims: The aim of this study was to investigate the combined effect of interval training and resveratrol consumption on the regulatory and executive factors of hepatocyte apoptosis in male rats with diabetes. Methods: Frothy-two male Wistar rats were randomly divided into healthy control, diabetic, saline diabetic, resveratrol supplement, intermittent exercise, and resveratrol+intermittent exercise groups. The interval training program consisted of ۸ weeks of training with an intensity of ۵۰%-۷۰% of the maximum oxygen consumption. Resveratrol was injected in two training groups with resveratrol supplement and resveratrol+supplement at a dose of ۲۰ mg. Results: The concentrations of caspase-۳ and BAX in diabetic saline and diabetic groups were significantly higher than in control groups (P=۰.۰۰۱). On the other hand, Bcl-۲ (B-cell lymphoma ۲) levels in diabetic saline and diabetic were significantly lower than in the control groups (P=۰.۰۰۱). The concentration of the BAX/Bcl-۲ ratio in diabetic and diabetic saline was significantly higher compared to control groups (P=۰.۰۰۱) The effect of interval training, administration of resveratrol alone, or especially with interval training caused a significant decrease in the concentration of BAX and caspase-۳ (P=۰.۰۰۱), a significant increase in the mean Bcl-۲ concentration (P=۰.۰۰۱), and a significant decrease in the mean BAX/Bcl-۲ ratio (P=۰.۰۰۱) in male rats with diabetes compared with the diabetic and diabetic groups. Conclusion: The combined effect of eight-week resveratrol supplementation and interval training decreased apoptosis markers, while it increased the concentration of Bcl-۲ in male rats with type ۲ diabetes.