Identification of Campylobacter Jejuni and Campylobacter Coli from Diarrheic Samples Using PCR

Background: Campylobacter jejune and C. coli are recognized as the most common bacteriological causes of gastroenteritis in humans. In this study Identification of Campylobacter Jejuni and Campylobacter Coli from samples using PCR was explored.Methods: Detection was performed using diarrheal samples collected ۸ from ۱۱۷ children. The genomic DNA of samples was extracted by phenol-chloroform method. All DNA extracts were examined for the presence of C.jejuni and C.coli species based on PCR method. Results: Of ۱۱۷ diarrheal samples, ۳۵ (۲۹.۹%) were found positive for ۱۰ Campylobacter spp using PCR. Conclusions: The results of this study showed that PCR is effective for rapidly screening stool samples for Campylobacter spp, due to its high sensitivity and specificity.Background: Campylobacter jejune and C. coli are recognized as the most common bacteriological causes of gastroenteritis in humans. In this study Identification of Campylobacter Jejuni and Campylobacter Coli from samples using PCR was explored. Methods: Detection was performed using diarrheal samples collected ۸ from ۱۱۷ children. The genomic DNA of samples was extracted by phenol-chloroform method. All DNA extracts were examined for the presence of C.jejuni and C.coli species based on PCR method. Results: Of ۱۱۷ diarrheal samples, ۳۵ (۲۹.۹%) were found positive for ۱۰ Campylobacter spp using PCR. Conclusions: The results of this study showed that PCR is effective for rapidly screening stool samples for Campylobacter spp, due to its high sensitivity and specificity.

Keywords:

Campylobacter jejuni , Campylobacter coli , Isolation , PCR.