Isolation and Identification of a Sewage Pseudomonas aeruginosa-infecting Bacteriophage
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JMMI-11-3_003
تاریخ نمایه سازی: 29 بهمن 1402
Abstract:
Introduction: Pseudomonas aeruginosa, a nosocomial pathogen, causes respiratory infections, particularly in individuals with cystic fibrosis (CF); it poses a significant challenge due to escalating antibiotic resistance. Phage therapy is emerging as a potential alternative approach to treating antibiotic-resistant bacteria. This study aimed to isolate and identify a highly efficacious bacteriophage targeting P. aeruginosa. Methods: Sewage samples from Mofid Children's Hospital were collected and titrated using a double-layer plaque assay. After plaque detection, the phage was isolated, and then the host range, adsorption rate, and stability were evaluated through spot tests and double-layer plaque assays. Phage morphology was determined using transmission electron microscopy (TEM). Results: Of the three sewage samples collected, one contained phage. We isolated a lytic bacteriophage that exhibited clear plaque formation and induced lysis in planktonic P. aeruginosa. TEM analysis revealed that the phage belonged to the Myoviridae family. The phage had a broad host range and demonstrated optimal stability at ۳۷ °C and pH ۷. Conclusion: The isolated phage can potentially be used for treating P. aeruginosa lung infections, offering an alternative approach to address drug-resistant isolates.
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Authors
Mahshid Setayesh Nia
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Ameneh Elikaei
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Ahya Abdi Ali
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Zeynab Rezaei
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
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