Investigating the Antimicrobial Properties of Synthetic Zinc Nanoparticles in the Medicinal Plant on Escherichia Coli causative of Bacteriuria in Pregnant Women

Background & Objective: Urinary tract infection is one of the most common asymptomatic infectious diseases during pregnancy, which is mostly caused by Escherichia coli infection, which, if not diagnosed and treated, causes harm to the mother and the fetus.Materials & Methods: In a cross-sectional study of ۵۰ pregnant women who referred to the outpatient clinic of Zabol hospital center after completing the questionnaire and examining the patients by obstetrician and gynecologist and ensuring that they were healthy, a sterile mid-stream urine sample was prepared and examined and diagnosed in terms of a complete urine test and placed were and Escherichia coli samples were separated from other colonies by diagnostic tests. The characterization of ZnO-NPs was performed by, Fourier-transform infrared spectroscopy, scanning and transmission electron microscopes, and X-ray diffraction.  The antimicrobial activity of ZnO-NPs synthesis in Eucalyptus was studied both in vitro against E. coli clinical isolates from woman.Result:The results of the analyzes showed that the zinc oxide nanoparticles were synthesized in a polyhedral to round shape and the size of the nanoparticles was ۲۱ nm. The results of the antibiotic resistance pattern investigation showed that the most sensitive to the antibiotic amikacin was and also the minimum inhibitory concentration of synthetic zinc nanoparticles was equal to ۱۵۰۰ μg/ml.Conclusion: In this study, it was found that aqueous extract of Eucalyptus leaves has the ability to produce zinc nanoparticles oxide nanoparticles have been synthesized from zinc sulfate, and the synthetic nanoparticles have shown good antimicrobial properties against antibiotic-resistant Escherichia coli bacteria.

Keywords:

Biosynthesis , Zinc , E. coli , Pregnant women , bacteriuria