Outer membrane Proteins-focused Pseudomonas aeruginosa Vaccine Designed using Reverse Vaccinology

Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJMM-17-5_008

تاریخ نمایه سازی: 30 بهمن 1402

Abstract:

Background and Aim: Antibiotic resistance is recognized as a major threat to global health that can result in increased morbidity and mortality. On February ۲۷, ۲۰۱۷, the first list of antibiotic-resistant priority pathogens was published by the World Health Organization (WHO). These pathogens, including Pseudomonas aeruginosa, pose the greatest threat to human health. This research was conducted in order to introduce viable candidate vaccine proteins against P. aeruginosa outer membrane proteins (OMPs) using reverse vaccinology approaches. Materials and Methods: Fifty-eight clinical isolates and ۹۹۸۲ outer membrane protein sequences were retrieved for vaxign۲ calculation of sequence-derived features. First, ۳۰ proteins with the highest adhesion probability were selected, and in the next step, ۱۰ candidates common among the ۵۸ strains with the highest scores were introduced as candidates for further studies. After determining the physicochemical characteristics of these vaccine candidates, the presence of protected domains was predicted in ۲ out of ۱۰ proteins. Results: Based on the results obtained from the bioinformatics analysis of the antigenic properties of these proteins, we identified ۱۰ outer membrane proteins that have the highest antigenic scores, are common among all ۵۸ clinical isolates, have no human protein homologs, and have less than ۱ transmembrane helix. These candidate proteins have optimal physicochemical properties. The presence of conserved domains was predicted only in the outer membrane porin F and enterobactin iron receptor. Conclusion: We suggested ۱۰ candidate proteins that showed suitable characteristics including outer membrane protein F (OprF) and ferric enterobactin receptor.

Authors

Masoumeh Fathi

Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Ebrahim Barzegari

Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences Kermanshah, Iran

Lida Lotfollahi

Department of Microbiology and Virology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

Reza Jafari

Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran

Bizhan Nomanpour

Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Mahsa Rasekhian

Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

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