Sertoli cell-conditioned medium can improve blood-testis-barrier function and spermatogenesis in azoospermia mice induced by scrotal hyperthermia: An experimental study

Background: An increase in the temperature of the testis is associated with damage to the epithelium of seminiferous tubules and disruption of sperm production. Objective: The current study aimed to investigate the effect of the Sertoli cell-conditioned medium (SCCM) on the blood-testis-barrier associated genes and spermatogenesis process following scrotal hyperthermia. Materials and Methods: In this experimental study, ۴۰ adult NMRI mice (۸ wk, ۲۵-۳۰ gr) were allocated into ۴ groups: I) control, II) DMEM (۱۰ μl Dulbecco's Modified Eagle Medium), III) scrotal hyperthermia, and IV) scrotal hyperthermia+SCCM (۱۰ μl SCCM). Hyperthermia was induced by placing the mice scrotum in water at ۴۳°C for ۲۰ min every other day for ۱۰ days. Mice were treated every other day for ۵ wk. Then the animals were euthanized, and the tails of epididymis were removed to analyze sperm parameters, testis were taken for stereological assessment, reactive oxygen spices and glutathione levels, and the expression of Ocln, Gja۱, Cdh۲, and Itgb۱. Results: The results of sperm analysis indicated that SCCM-treated mice significantly increased sperm count and motility and reduced DNA fragmentation. In addition, histological and molecular findings showed that the volume of testicular tissue, the number of germ cells, the glutathione level, and the expression of Ocln, Gja۱, Cdh۲, and Itgb۱ genes were significantly increased in the SCCM-treated mice. Conclusion: Findings suggest that growth factors of SCCM stimulate the proliferation and differentiation of germ cells through paracrine effects and upregulate the blood-testis-barrier-associated genes in mice subjected to scrotal hyperthermia.

Keywords:

Testis , Sertoli cells , Culture media , Hyperthermia , Spermatogenesis. , بیضه , سلول سرتولی , محیط کشت , هیپرترمی , اسپرماتوژنز.