Laboratory Comparison of the Anti-Bacterial Effect of Prosopis Farcta Extract and Hypochlorite Sodium on Enterococcus Faecalis Bacteria

Background and Aim: The aim of root canal therapy is to eliminate intra canal irritants and prevent the reinfection of root canal. The purpose of the present study was to compare laboratory antimicrobial effect of Prosopis farcta extract with ۵.۲۵ % hypochlorite against E.faecalis. Materials and methods: In this experimental laboratory study two solutions of ۵.۲۵% hypochlorite and seeds extract of Prosopis farcta after preparation were adjoining to oral pathogen E.faecalis. Antibacterial effect using disc diffusion and MIC method with serial dilution was assessed. The mean values of inhibition zone around discs in Mueller- Hinton agar plates were measured and compared using a ruler. Each test repeated ۳ times. Data were analyzed applying SPSS (ver ۱۷) and Kruskal-Wallis statistical test. Results: There were significant difference in mean value of inhibition zone between Prosopis farcta extract and ۵.۲۵ % hypochlorite (P >۰.۰۰۰۱). Bacterial growth inhibition was assessed in the MIC and MBC test, as the reported MIC was higher than ۵۱۲ mg / ml because the bacteria had grown in all test tubes. Conclusion: Considering the limitations of a laboratory study, it seems that Prosopis farcta seeds extract in comparison to ۵.۲۵ % hypochlorite has no antibacterial effect on E.faecalis.

Keywords:

hypochlorite sodium , Prosopis farcta plant , root canal irrigation , E.faecalis , هیپوکلریت سدیم , گیاه کهورک , شستشوی کانال ریشه , انتروکوکوس فکالیس