Photosensitivity and Radiosensitivity of Indocyanine Green on Human Cell Lines MCF۷ and DFW

Background & Aims: In this study with the aim of benefiting from non-laser sources in photodynamic therapy, photo and radio sensitivity of indocyanine green as a sensitizer in photodynamic and radiation therapies were investigated. Methods: Based on the broad absorption peak of indocyanine green and using non-coherent light, the experiments were performed on human cells derived from breast cancer and melanoma. To investigate chemical, optical and radiational cytotoxicity and also photosensitivity and radiosensitivity of indocyanine green, different dozes of this material were examined. After ۲۴ h of incubation of cells with indocyanine green, independent treatment groups were exposed to ۷۳۰±۲۰ nm light with power densities of ۳۰, ۶۰, and ۱۰۸ J/cm۲ and ۱۰۰ kVp x-ray (۲ and ۴ Gy). The effect of therapy on cells was determined by MTT test. Results: Indocyanine green showed no significant cytotoxicity. It had a good efficiency for photodynamic therapy using non-coherent sources in the wavelength of ۷۳۰±۲۰ nm, and the efficiency of treatment was dependent on the dosage of light. No significant relation between indocyanine green and radiation was observed. Conclusion: According to the findings, indocyanine green can be used as a photosensitizer in the range of ۷۳۰±۲۰ nm. Since there was no significant difference between groups which received both radiation and drug and those which received only radiation, indocyanine green cannot be considered as a radiosensitizer.

Keywords:

Photodynamic therapy , Breast Neoplasms , Melanoma , Indocyanine Green , Radio sensitivity Non-coherent