Study of Anti-Retroviral Effects of Salix Aegyptiaca L Herbal Extract on HIV-۱ in-vitro

Human immunodeficiency virus (HIV) infection is the major problem in the world. Nowadays, new anti-retroviral drugs are extracted from medicinal plants, make an important role to supersede synthetic drugs with different side effects. In this study, we studied the Salix aegyptiaca L extract, Iranian herb, as an anti-HIV drug with anti-retroviral effects. We used a single cycle replicable (SCR) HIV-۱ system by co-transfection of HEK ۲۹۳T with pmzNL۴-۳, psPAX۲ and pM۲G.۲. Cytotoxicity and cytopathic protection assay were performed by XTT method. Inhibition of p۲۴ Ag production level assay was done using quantitative enzyme linked immunosorbent assay (ELISA). Salix aegyptiaca L inhibited HIV-۱ induced CPE in HELA cells with SI value of ۲۲.۲. Finally, we use informatics analysis such as Gaussian, Hyper Chem , HF and B۳LYP methods and ۶-۳۱G, ۶-۳۱G** and ۶-۳۱۱G** basis sets. These results suggest that Salix aegyptiaca L has potent anti-HIV activity and may be a promising candidate for AIDS.

Keywords:

Human immunodeficiency virus۱ , Salix aegyptiaca L , single cycle replicable virion (SCR) , Gaussian , HF , Hyper Chem