Effects of Citrus limon (L.) essential oil on Pseudomonas aeruginosa: complemented with a computational approach; focus on quorum sensing

Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
View: 30

This Paper With 17 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_IJMCM-10-1_005

تاریخ نمایه سازی: 18 اسفند 1402

Abstract:

Antibiotic-resistance is becoming an increasingly important concern. Resistant bacteria like Pseudomonas aeruginosa have different strategies while encountering either the immune system or antibiotics. As an example biofilm formation or quorum sensing can be mentioned as bacterial mechanisms to gain resistance. Therefore, it is crucial to explore novel compounds that might disrupt biofilm production especially using organic and plant-based compounds. Citrus limon essential oil has been used against multiple types of microorganisms in traditional medicine. The purpose of this study is to investigate the effect of this essential oil on P. aeruginosa biofilm formation. The inhibition of biofilm was measured using a micro-dilution method, staining the biofilm that has been formed and determining the minimum inhibitory concentration that proved effective. Then, via molecular docking software, the ligands more likely to interact with biofilm proteins were predicted. Our results suggest that after multiple determinations of minimum inhibitory concentration, the lowest effective dose was in concentrations between ۱۰۰ µg/ml to around ۴۵۰ µg/ml. Furthermore, molecular docking results demonstrated that between all the essential oil ligands, the top three were geranyl acetate, α-terpineol, and β-bisabolene. To sum up, Citrus limon essential oil showed promising anti-quorum sensing properties and it can be considered as a source of antimicrobial formulation.

Authors

Arghavan Arjmandi

Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran

Khosrow Chehri

Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran,

Isaac Karimi

Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran