Examining the antibacterial activity of Artemisia dracunculus L. extract in various extracting methods

Background and purpose: The status of medicinal plants in Iranian traditional medicine and rich existence of herbal sources, on one hand, and current problems in curing infections, on the other hand, are the factors to study medicinal plants more accurately. Tarragon (Artemisia dracunculus L.) is a herbaceous plant from Asteraceae family, available in planted and farming forms in Iran. This study aims to examine the antibacterial activity of Artemisia dracunculus L. in various extraction methods.Method: The Maceration and Soxhlet methods were used to Methanolic extract of Artemisia dracunculus L. the antibacterial effect of extracts was conducted in various concentrations on Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa microorganisms by Disk diffusion and Well Diffusion, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) methods. Findings: the results indicated that both extracts obtained by Maceration and Soxhlet methods had antibacterial effects just on two gram-positive bacteria, Staphylococcus aureus and Bacillus subtilis. Both gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa, were resistant to the extracts in all concentrations.

Keywords:

Artemisia dracunculus L , antibacterial activity , exatrcting methods , disk diffusion , Well Diffusion