The study of Cumin oil essence on the function of FUM۱ genes in Fusarium verticillioides by molecular analysis

Fusarium is one of the most important pathogens of cereals; the fungal infection has increased in recent years due to ecological changes and soil nitrogen content increase. In the current study the effect of cumin oil essence on fusarium verticillioides by molecular method was investigated. The study included ۱۴ isolates, ۱۱ isolates of fusarium verticillioides and ۳ Fusarium non verticillioides samples (negative control) were tested. For testing MIC and MFC, the RPMI۱۶۴۰ culture in microplate was used. After a week, the cultured RNA samples using the RNA extraction kit of Fermentas company (Purification Mini Kit GeneJET Plant RNA) was extracted from fungal biomass and the RT-PCR test along with the FUM۱ gene was performed. The RT-PCR analysis displayed that FUM۱ gene was expressed in fungal samples that were not under the effects of oil essence, but the gene was not expressed in samples after the cumin impact and there was not any band in agarose gel. the B۱ fumonisin toxin which was produced by the fungus fusarium verticillioides contains several genes. The gene FUM۱was involved toxin gene synthesis pathway of this toxin as the most essential gene.

Keywords:

Fusarium verticillioides , Fumonisin , cumin , FUM۱ , ITS