Isolation and Identification of extremophiles producing anticancer L-glutaminase and L- Methioninas enzymes from Yale Gonbad hot spring in Qazvin, Iran
Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJMCM-8-2_007
تاریخ نمایه سازی: 19 اسفند 1402
Abstract:
L Glutaminase and L- methioninase, therapeutic enzyme obtained from microbial source have been considered in the treatment of cancer. A total ۲۰ water samples were obtained from the Yale Gonbad hot spring in Qazvin province, Iran. Production capabilities L Glutaminase and L- methioninase were tested on m۹ and mcd plates, respectively. Enzyme-producing isolates were identified by conventional test and then confirmed using molecular methods. The PCR amplicons were directed for sequencing and Phylogenetic tree were drawn using Mega version ۶ software. Twelve colonies were obtained from all tested samples. Three extratermophilic isolates showed L-glutaminase activity in M۹ medium but only ۱ isolates have L-methioninase activity in MCD medium. The phylogenetic tree drawn from the ۱۶ S rRNA gene sequence showed were ۱۰۰% similar to B. licheniformis and strains of S. epidermidis and L. sphaericus. In this study, Bacillus licheniformis was identified as a dominant and a native strain for the production of L-glutaminase and L-methioninase.
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Authors
Mahnaz Farahmand
Department of biology, East Tehran branch, Islamic Azad University, Tehran, Iran
Elham Azizi
Department of biology, East Tehran branch, Islamic Azad University, Tehran, Iran
Mohammad Hassan Shahhosseini
Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran