Isolation and Identification of extremophiles producing anticancer L-glutaminase and L- Methioninas enzymes from Yale Gonbad hot spring in Qazvin, Iran

L Glutaminase and L- methioninase, therapeutic enzyme obtained from microbial source have been considered in the treatment of cancer. A total ۲۰ water samples were obtained from the Yale Gonbad hot spring in Qazvin province, Iran. Production capabilities L Glutaminase and L- methioninase were tested on m۹ and mcd plates, respectively. Enzyme-producing isolates were identified by conventional test and then confirmed using molecular methods. The PCR amplicons were directed for sequencing and Phylogenetic tree were drawn using Mega version ۶ software. Twelve colonies were obtained from all tested samples. Three extratermophilic isolates showed L-glutaminase activity in M۹ medium but only ۱ isolates have L-methioninase activity in MCD medium. The phylogenetic tree drawn from the ۱۶ S rRNA gene sequence showed were ۱۰۰% similar to B. licheniformis and strains of S. epidermidis and L. sphaericus. In this study, Bacillus licheniformis was identified as a dominant and a native strain for the production of L-glutaminase and L-methioninase.

Keywords:

extremophilic bacteria , L-glutaminase , L- Methioninas , anticancer , Iran