A Study on the Antimicrobial Effect of Zinc Oxide Nanoparticles on Clinical Strains of Staphylococcus aureus Resistant to Vancomycin

Staphylococcus aureus, as one of the main agentsfor hospital infections,is considered as highly important because they show resistance to a wide range of antibiotics.Resistance to selective antibiotics such as vancomycin is a serious problemin the medical community; thus it seems rational to use alternative substances for treating these bacteria.The aim of this study isto investigate the prevalence of resistance to vancomycin in clinical isolates of Staphylococcus aureus as well as theantimicrobial effects of zinc oxidenanoparticles on them. In this study, ۷۰ samples of wound, boil, abscess and urine were isolated.Staphylococcus aureus strains resistant to vancomycin were then identified through routine laboratory tests using Broth Microdilution test. The antibacterial effect of ZnO nanoparticles(۲۰ nanometer) was investigated at concentrations of ۱۰۰,۵۰,۲۵,۱۲.۵ and ۶.۲۵ mg/ml using Agar well diffusion method over strains resistant to vancomycin. From the total of ۷۰ samples, ۳۰ samples were identified as Staphylococcus aureus out of which ۲۳.۳% showed resistance to vancomycin. During this study it was found that ZnO nanoparticles in concentrations of ۵۰ and ۱۰۰ mg/ml have a good antibacterial effect and can be a good alternative for controlling Staphylococcus aureus resistant to vancomycin. Considering the increasing trend in drug resistance, the growth of pathogenic bacteria can be inhibited by increasing the concentration of zinc oxide nanoparticles.

Keywords:

Staphylococcus aureus , Vancomycin , Zinc Oxide , Nanoparticle , Drug resistance