Molecular identification of Azotobacter isolated from the rhizosphere of Karunvariety of barley in desert and its comparison with standard Azotobacter andinvestigation of some of the vegetation indicators after inoculation
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJMCM-6-2_003
تاریخ نمایه سازی: 19 اسفند 1402
Abstract:
This study was conducted in acompletely randomized block design with three replications using three concentrations of ۱۰۷, ۱۰۵, and ۱۰۳cfu/ml of Azotobacter isolated from the rhizosphere of Karun varieties of barley (Az. salentris) and standard Az.chrococom along with control (zero concentration of bacteria). The aim of this study was to study the reactions of barley plantin response to inoculation by mentioned bacteria in the form of infected separate inoculation and non-inoculated seeds as a control. For isolation the number of mentioned bacteria, Karun variety of barley in desert was cultivated in the mannitol broth environment, and macroscopic and microscopic characteristics of bacterial colonies were evaluated. Finally, using gene ۱۶Sr DNA, isolated variety was identified molecularly. At the end of plant growth, important traits such as percent of germination, total protein level, and activity of antioxidant enzymes of catalase and peroxide were measured from the desired plant and they were analyzed statistically. After ۱۸ days, results showed that the concentration of ۱۰۷, ۱۰۵, ۱۰۳cfu / ml of Azotobacter isolated from the rhizosphereKarun variety of barley plant in the desert (Az. salentris) increased the activity of antioxidant enzymes, total protein, and percentage of germination compared with control. In addition, different concentrations of Azotobacter isolated from the rhizosphere of barley plant (Az. salentris) showed higher and significant impact on catalase activity, total protein level, and percentage of Karun variety of barley germination compared with standard Az.chrococom.
Keywords:
Antioxidant enzymes , total protein , Azotobacter , Colony - PCR , germination , Karun variety of Barley
Authors
zahra alsadat mirmohamadi
M.Sc,Department of Microbiology,Falavarjan Branch,Islamic Azad University,Falavarjan,Isfahan,Iran
Monir Doudi
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Sima Yahyaabadi
Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.