Estrogen treatment enhances neurogenic differentiation of human adipose derived stem cells in vitro
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 18، Issue: 8
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
View: 34
This Paper With 6 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-18-8_010
تاریخ نمایه سازی: 23 اسفند 1402
Abstract:
Objective(s):Estrogen is a sexual hormone that has prominent effects on reproductive and non-reproductive tissues. The aim of this study is to evaluate the effects of estrogen on the proliferation and neural differentiation of human adipose derived stem cells (ADSCs) during neurogenic differentiation. Materials and Methods: Isolated human ADSCs were trans-differentiated in neural induction medium containing neurobasal medium, N۲ and B۲۷ with or without ۱۷β-estradiol (E۲) treatment. Proliferation rate and neural differentiation of human ADSCs were assessed using MTT assay, immunostaining and real time RT- PCR analysis, respectively. Results: Analysis of data show that estradiol treatment can significantly increase proliferation rate of differentiated cells (P<۰.۰۵). Immunocytochemical and real time RT-PCR analysis revealed that the expression of precursor and mature neuronal markers (nestin and MAP۲) was significantly higher in the E۲ treated cell cultures when compared to the untreated cell cultures (P<۰.۰۵). Conclusion: According to our findings, estrogen can promote proliferation and neuronal differentiation of human ADSCs.
Keywords:
Authors
Shahnaz Razavi
Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran
Mohamad Reza Razavi
Molecular Parasitology Laboratory, Pasteur Institute of Iran, Tehran, Iran
Nafiseh Ahmadi
Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran
Mohammad Kazemi
Department of Genetic, Isfahan University of Medical Sciences, Isfahan, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :