Decellularized kidney in the presence of chondroitin sulfate as a natural ۳D scaffold for stem cells
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 18، Issue: 8
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
View: 33
This Paper With 11 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-18-8_009
تاریخ نمایه سازی: 23 اسفند 1402
Abstract:
Objective(s): Use of biological scaffolds and automating the cells directing process with materials such as growth factors and glycosaminoglycans (GAGs) in a certain path may have beneficial effects in tissue engineering and regenerative medicine in future. In this research, chondroitin sulfate sodium was used for impregnation of the scaffolds. It is a critical component in extracellular matrix and plays an important role in signaling pathway; however, little is known about its role within mammalian development and cell linage specification. Materials and Methods: Due to its porous and appropriate structure and for putting cells in ۳D space, the kidney of BALB/c mouse was selected and decellulalized using physical and chemical methods. After decellularization, the scaffold was impregnated in chondroitin sulfate solution (CS) for ۲۴ hr. Then, ۶۰×۱۰ ۵ human adipose-derived mesenchymal stem cells were seeded on the scaffold to assess their behavior on day ۵, ۱۰, ۱۵, ۲۰, and ۲۵. Result: After ۴۸ hr, DAPI staining approved completed decellularized kidney by ۱% SDS (sodium dodecyl sulfate). Migration and establishment of a number of cells to the remaining area of the glomerulus was observed. In addition, cell accumulation on the scaffold surface as well as cells migration to the depth of kidney formed an epithelium-like structure. Up to the day ۱۵, microscopic study of different days of seeding showed the gradual adhesion of large number of cells to the scaffold. Conclusion: Glycosaminoglycan could be a right option for impregnation. It is used for smartification and strengthening of natural scaffolds and induction of some behaviors in stem cells.
Keywords:
Authors
Alireza Rafighdoust
Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Nasser Mahdavi Shahri
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
Javad Baharara
Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :