Effects of Intraperitoneal Administration of Polyethylene Glycol Solution on Ischemia-Reperfusion Injury in Rat Testicular Torsion and Detorsion Model

Testicular torsion and detorsion are significant clinical issues for infertile men. Torsion of the spermatic cord is an emergency condition resulting from the rotation of the testis and epididymis around the axis of the spermatic cord. A rat testis model was used to assess the effects of polyethylene glycol on ischemia-reperfusion injury. Twenty-four healthy male Wistar rats were used. The rats were included and randomized into four investigational groups (n = ۶): Group Sham: Merely laparotomy was implemented. Group Ischemia: Merely ۳-hour interval ischemia was done. Group IS/REP: A ۳-hour interval ischemia, ۳-hour reperfusion for left testis, one-week reperfusion for right testis were done and ۲۰ µl normal saline was administered intraperitoneally (IP) ۳۰ min before termination of ischemia. Group IS/REP/PEG: The same as group IS/REP as well as ۲۰ µl PEG solution ۳% (IP) ۳۰ min before termination of ischemia. Evaluations were based on biochemical analyses and sperm parameters morphometry. Polyethylene glycol enhanced antioxidant activity and quality of sperm parameters (p < ۰.۰۵). In conclusion, polyethylene glycol could be helpful in minimizing ischemia-reperfusion injury in testicular tissue exposed to ischemia

Keywords:

polyethylene glycol , ischemia-reperfusion , Intraperitoneal , Testis