Comparative Evaluation of Standing and Lateral Recumbency Restraint Positions for Rumenotomy Based on Transforming Growth Factor-β Responses in Kano-Brown Goats

Objective- Comparative evaluation of standing and lateral recumbent restraint positions for rumenotomy based on transforming growth factor-β (TGF-β) concentrations of Kano-Brown goats (KBGs).Design- Experimental studyAnimals- Eighteen KBGs of both sexes diagnosed of rumen foreign body impaction (RFBI), were allocated to groups A, B and D. Six other KBGs free of RFBI were assigned to group C as control.Procedures- Groups A and B were restrained in lateral recumbency position while group D in a fabricated mobile small ruminant surgical chute (MSRSC) in a standing fashion. Serum samples stored at -۲۰ ℃ until ELISA, were obtained pre-rumenotomy (Pre) and post-rumenotomy, at ۰, ۵, ۲۴, ۴۸ and ۷۲ hours, and subsequently at weeks ۱, ۲, and ۳. Group C had no surgery while A, B and D had rumen skin clamp fixation, stay suture rumenotomy and mobile small ruminant surgical chute rumenotomy, respectively. Results- The post-rumenotomy mean concentrations of TGF-β for groups A, B and D at ۰ hour (۸۱.۹۷ ± ۲۴.۱۲, ۷۱.۲۶ ± ۱۰.۲۸ and ۵۸.۵۱ ± ۶.۴۴ ng/L, respectively) were higher than the mean pre-rumenotomy values (۳۸.۳۴ ± ۳.۶۶, ۴۱.۳۱ ± ۴.۹۰ and ۴۴.۹۱ ±۴.۱۰ ng/L, respectively) but were not significantly different (P > ۰.۰۵). As the mean TGF-β concentration in the males of the different experimental groups did not differ significantly (P > ۰.۰۵), the females of group B had significantly higher (P < ۰.۰۵) mean concentrations than those of group D and C females at ۴۸ hours post-rumenotomy. Conclusions and Clinical Relevance- Lateral recumbency restraint position rumenotomy was associated with more severe post-surgical stress than standing restraint based on role switching of the TGF-β in this study. This suggests comparative advantage of standing recumbency restraint rumenotomy over the conventional lateral recumbency restraints position in goats.

Keywords:

Surgical stress , Caprine , Rumen , Chute , Cytokines , ELISA