An in vitro antiviral evaluation of punicalagin toward influenza A virus
Publish place: Avicenna Journal of Phytomedicine، Vol: 14، Issue: 4
Publish Year: 1403
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_AJP-14-4_007
تاریخ نمایه سازی: 23 خرداد 1403
Abstract:
Objective: Influenza complications are mild to serious, and cancause death in some cases. A great deal of attention has been paidin recent years to the development and use of new antiviralcompounds to overcome drug resistance in certain strains of theinfluenza virus and treat the clinical implications. This studyaimedto investigate the antiviral effect of punicalagin and itsassociatedmechanism against influenza A (H۱N۱) virus in vitro.Materials and Methods: the ant-influenza activity of punicalaginwas studied in Madin-Darby Canine Kidney (MDCK) cells usinginfluenza virus A/Puerto Rico/۸/۳۴ (H۱N۱) (PR۸) usingHemagglutinin assay (HA) and ۵۰% tissue culture infective dose(TCID۵۰). Then, the inhibition of haemagglutination, virucidalactivity, inhibitory effect at different times, replication of viralRNA and expression of viral genes were investigated.Results: Punicalagin could inhibit influenza virus infection with۵۰% inhibitory concentration (IC۵۰) of ۳.۹۸ μg/ml and selectivityindex (SI) value of ۶.۱. Punicalagin decreased virus titers with aninhibitory effect on virus hemagglutination (p<۰.۰۵). Punicalaginalso inhibited viral adsorption. The results of virus RNAreplicationand viral mRNA (NS۱ and HA) expression aftertreatment withpunicalagin showed significant suppression of viral mRNAexpression but no effect on replication of viral RNA.Conclusion: The results of the present study indicated thatpunicalagin was effective against influenza infection mostprobablyvia inhibition of haemagglutination activity and virusbinding.
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Authors
Fatemeh Javadi-Farsani
Student Research Committee, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
Ali Karimi
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Hadi Razavi Nikoo
Department of Microbiology and Virology, Golestan University of Medical Sciences, Gorgan, Iran
Mohammad-Taghi Moradi
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Alijan Tabarraei
Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran