Genetic polymorphism of -Lg gene in Iraqi buffalo using Polymerase Chain Reaction – Restriction Fragment Length Polymorphism
Publish place: Caspian Journal of Enviromental Sciences، Vol: 21، Issue: 2
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_CJES-21-2_009
تاریخ نمایه سازی: 31 خرداد 1403
Abstract:
This study was conducted on ۵۰ buffaloes from Basrah Governorate, Iraq to study the genetic polymorphisms of the β-lactoglobulin gene in the local buffaloes. The lab examinations were conducted in the Genetic Engineering Laboratory, College of Agriculture, University of Basrah. DNA was extracted by kit (PureLink Genomic DNA kits, Invetrogen, USA). The results of this technique showed the appearance of the expected gene bundle of ۲۵۲ base pairs from all Iraqi buffalo samples, as the primer interacted with all DNA isolates taken from the studied buffalo samples. Digestion technique with restriction enzymes (HaeIII) was used to detect the genetic polymorphisms of β-lactoglobulin gene, exhibiting two bands to all studied buffalo samples. Only the homozygous BB genotype has been shown.
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Authors
Bashar F. Zaqeer
College of Agriculture, University of Basrah, Basrah, Iraq
Muntaha Y. Yousief
College of Agriculture, University of Basrah, Basrah, Iraq
Salah Mahdi Alsudany
College of Agriculture, University of Basrah, Basrah, Iraq
Jaafar M. Owaid
College of Agriculture, University of Basrah, Basrah, Iraq
Falih Hasan Hamad
College of Agriculture, University of Basrah, Basrah, Iraq
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