Design And Fabrication Of Microfluidic Real-Time PCR Chip On Glass Substrate For Rapid Detection

DNA replication in the body is necessary for the construction and survival of an organism. One of the phenomenal phenomena related to the discussion is that with the advent and expansion of the science of polymerase chain reaction (PCR), researchers could reach a tremendous triumph in DNA amplification outside the body and detecting a specific sequence in DNA. This process requires ۳ stages denaturation, annealing, and extension, each needing a specific temperature region. This research, with the goals of the accuracy of diagnosis and improving microfluidic channels and heat transfer, plays a vital role in performing a more applicable real-time polymerase chain reaction (real-time PCR). We developed a new design of beneficial microfluidic channels on a glass chip and brought it to reality by photolithography and chemical etching methods. We also designed an electronic system to act as a processor core, a controller, and a display for real-time information. It would help us achieve all the temperatures required for the process, the light stimulation part, and the light detection sensor. In this project, we used Syber green real-time PCR master mix and Glyceraldehyde ۳-phosphate dehydrogenase (GAPDH) primer and synthesized the complementary DNA (cDNA) of the cancer cell, we were able to inject our solution into the microchannels with using mineral oil flow and we were able to multiply the GAPDH gene in the cancer cell.

Keywords:

Micro Channel , Real-time PCR , Rapid detection , Lab on a Chip , DNA