Assessment of the Microbiome Role in Skin Protection Against UV Irradiation Via Network Analysis
Publish place: Journal of Lasers in Medical Sciences، Vol: 11، Issue: 3
Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JLMS-11-3_001
تاریخ نمایه سازی: 22 مرداد 1403
Abstract:
Abstract Introduction: Diverse microbiotas which have some contributions to gene expression reside in human skin. To identify the protective role of the skin microbiome against UV exposure, protein-protein interaction (PPI) network analysis is used to assess gene expression alteration.Methods: A microarray dataset, GEO accession number GSE۱۱۷۳۵۹, was considered in this respect. Differentially expressed genes (DEGs) in the germ-free (GF) and specific pathogen-free (SPF) groups are analyzed by GEO۲R. The top significant DEGs were assigned for network analysis via Cytoscape ۳.۷.۲ and its applications.Results: A total of ۲۸ genes were identified as significant DEGs and the centrality analysis of the network indicated that only one of the seven hub-bottlenecks was from queried genes. The gene ontology analysis of Il۶, Cxcl۲, Cxcl۱, TNF, Il۱۰, Cxcl۱۰, and Mmp۹ showed that the crucial genes were highly enriched in the immune system.Conclusion: The skin microbiome plays a significant role in the protection of the skin against UV irradiation and the role of TNF and IL۶ is prominent in this regard. Keywords: Microbiome UV radiation Gene expression Protein-protein interaction network