Extremely Low Frequency Electromagnetic Fields Alter Expression of C-Myc and circ-CCDC۶۶ in Gastric Cancer Cell Line

The biological effects of electromagnetic fields (EMF), in particular their beneficial or adverse impacts on the promotion and progression of cancer, have attracted considerable attention. C-Myc has a critical regulatory role in cell transformation and causes metabolic changes, that accompany malignant transformation. Observations have shown that c-Myc expression can be altered by circular RNAs (circRNA). In this study, the changes in the expression of c-Myc and circ-CCDC۶۶ in the AGS cell line after the extremely low-frequency magnetic fields (ELF-MF) exposure were investigated. The AGS cells were exposed to different magnetic flux densities for ۱۸ h, continuously and discontinuously (۲h on/۲h off). To evaluate the expression changes of c-Myc and circ-CCDC۶۶ real-time PCR was used. The results showed that discontinuous magnetic fields could reduce c-Myc expression. Continuous exposure of ۰.۲۵ mT could reduce the c-Myc expression, but with increasing the magnetic flux density c-Myc was upregulated. The expression level of circ-CCDC۶۶ decreased under exposure to continuous and discontinuous ELF-MFs. The difference between the results of c-Myc under the influence of magnetic fields may be described by the hypothesis of the window effect of the fields. Our results indicated that ELF-MFs can induce changes in c-Myc and circ-CCDC۶۶ expression.