β-carotene Has the Neuroprotective Effects in Parkinson’s Disease by Regulating Mitochondrial Apoptotic Pathway Genes
Publish place: Human Genetics and Genomics، Vol: 4، Issue: 2
Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JHGG-4-2_003
تاریخ نمایه سازی: 1 شهریور 1403
Abstract:
Background: Parkinson’s disease (PD) is one of the most common neurodegenerative diseases that cause disability. Finding treatment options that have no side effects can be very important.
Objectives: Therefore, in this study, the effect of β-carotene administration was investigated in the PD model of rats.
Methods: Induction of Parkinson’s disease in rats was done by injection of ۶-hydroxydopamine (۶-OHDA) into the substantia nigra (SN). After induction rat behaviour was studied using an apomorphine-induced rotation test. The SN cells’ viability was evaluated by MTT assay and apoptosis and necrosis were measured by flow cytometry. The expressions of bax and bcl-۲ genes were also studied using RT-PCR technique. Data analysis was done by GraphPad Prism V.۸ software.
Results: The results showed a positive effect of β-carotene administration in PD rats, which led to improvement in apomorphineinduced rotation test, increased viability, and decreased apoptosis and necrosis of SN neurons. It also downregulated bax and overexpressed bcl-۲ gene expressions (P < ۰.۰۱).
Conclusions: β-carotene has therapeutic effects in PD conditions and its mechanism of action was attributed to regulating the expressions of genes involved in mitochondrial apoptosis. Therefore, its study in a clinical setting is recommended.