Evaluation of changes in expression of muc۱۹ and muc۲۱ genes in colorectal cancer tissues

Colorectal cancer is the third most common cause of cancer deaths in the world. Mucins are large glycoproteins that are expresses by epithelial cells in tubular organs. They recreate important functions in the regeneration and differentiation of epithelium, cell adhesion, immune response, and cellular signaling. In this study, mucin-۱۹ and ۲۱ gene expression were investigated in cancerous tumor samples, and tumor margins in patients with colorectal cancer. The purpose of working on these genes is their potential biomarker for the diagnosis of colorectal cancer. After extraction of RNA from ۳۰ pairs of tumor and peripheral tumors of the colorectal tumor, cDNA was synthesized using a thermosetting kit, and RT-PCR was used to find the optimum temperature and specificity of primers. Then, real-time qPCR was performed to examine the difference between the gene expression of mucin on the tumor and tumor margins. This study showed an increase in the expression of mucin ۱۹ in colorectal cancer tissue samples with tumor margins. Conclusion: The upregulation levels of mucin ۱۹ gene can correlate with colorectal cancer progression. Therefore, Mucin ۱۹ may be used as a biomarker for detecting colorectal cancer development.

Keywords:

Colorectal cancer , Biomarker , Mucins , Muc۱۹ gene