Angiotensinogen Gene M۲۳۵T and T۱۷۴M Polymorphisms in Diabetic Nephropathy in a Bangladeshi Population

Background: Marker gene polymorphisms linked with the renin-angiotensin-aldosterone system (RAAS) have been broadly studied in diabetic nephropathy (DN) patients considering that RAAS is a potential drug target to slow down kidney disease progression. Objectives: The aim of the present study was to determine the link between M۲۳۵T and T۱۷۴M variants of angiotensinogen (AGT) gene and DN. Methods: A total of ۹۳ patients with DN, mean age of ۵۶±۸ years, systolic blood pressure (SBP) of ۱۴۱±۱۴, and diastolic blood pressure (DBP) of ۸۴±۷ mm Hg (mean±SD) were investigated, among whom ۵۹ patients had a family history of type ۲ diabetes mellitus. A total of ۹۶ healthy subjects served as the control group with no family history of diabetic nephropathy (FHDN) and type ۲ diabetes mellitus, a mean age of ۴۷±۱۰ years, SBP of ۱۲۶±۱۱, and DBP of ۷۶±۶ mm Hg. PCR–restriction fragment length polymorphism was employed for genotyping M۲۳۵T and T۱۷۴M molecular variants. Results: Genotype frequencies of the variants M۲۳۵T (χ۲=۲.۰۳۸, P=۰.۳۶۱) and T۱۷۴M (χ۲=۲.۹۵۲, P=۰.۲۲۹) did not show any statistically significant association with type ۲ diabetic nephropathy (T۲DN) compared to the control. Based on FHDN and family history of diabetes mellitus (FHDM), the frequency of genotypes of M۲۳۵T marker (P=۰.۳۶۰) in FHDN, and (P=۰.۸۸۶) FHDM; T۱۷۴M marker (P=۰.۶۴۱) in FHDN, and (P=۰.۴۲۵) FHDM also did not show any statistically significant association with T۲DN compared to the controls. Conclusion: M۲۳۵T and T۱۷۴M variants were not associated with DN in a Bangladeshi population.