Isolation and Molecular Identification of Acinetobacter baumannii From Urinary Tract Infection in Diyala Province, Iraq

Background and Objective: Acinetobacter baumannii is one of the most prominent opportunistic bacterial pathogens associated with hospital-acquired infections and has been associated with antibiotic resistance. The high rates of resistance have made it difficult to choose the appropriate treatment and put the lives of infected patients at risk of death. This study aimed to isolate A. baumannii from urinary tract infections (UTI) and detect the bacterial ability to form biofilms from clinical samples. Methods: In this study, A. baumannii bacteria were isolated from several sources (UTI). The microtiter plate method revealed biofilm formation. Clinical specimens were grown on selective media. The A. baumannii was identified by classical techniques; the VITECK combined 2 system and 16S rRNA gene amplification. Results: From the 130 suspected isolates, 20 isolates were obtained from A. baumannii multidrug-resistant (MDR) and extensively drug-resistant (XDR) types. Among them, 14 (70%) were MDR and 6 (30%) were XDR types. Conclusion: The results showed that A. baumannii bacteria were more resistant to antibiotics and had strong biofilm formation.

Isolation and Molecular Identification of Acinetobacter baumannii From Urinary Tract Infection in Diyala Province, Iraq Keywords:

Acinetobacter baumannii , Antibiotic resistant , Biofilm , Extensively drug-resistant , Multidrug-resistant