Association of Hsa-mir-196 and Hsa-mir-153 Expression Levels With Gastric Cancer and H. pylori Infection

Background and Aim: Helicobacter pylori (H. pylori) is a bacterium that plays a significant role in the development of gastric cancer (GC). It is estimated that H. pylori infection is responsible for more than 70% of all gastric cancer cases worldwide. By analyzing publicly available gene expression datasets, we aimed to evaluate differentially expressed genes (DEGs) linked to the GC and H. pylori infection. Materials and Methods: In this case-control study, total RNA was extracted from blood samples of 40 GC patients with 29 H. pylori positive samples, and 40 healthy controls with 25 H. pylori positive individuals. The quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression levels of hsa-mir-196 and hsa-mir-153. Results: Statistical analysis revealed a significant upregulation of hsa-mir-196 and a significant downregulation of hsa-mir-153 in the GC patients with H. pylori infection compared to those without infection and healthy controls. Conclusion: In-silico analysis demonstrated the association of hsa-mir-196 and hsa-mir-153 with GC. These miRNAs are potential biomarkers for the GC associated with H. pylori infection and can serve as diagnostic tools for the early detection and may have prognostic value in predicting disease progression. However, further validation in larger cohorts and functional studies are necessary to fully understand their role in the GC development and assess their potential as therapeutic targets.

Association of Hsa-mir-196 and Hsa-mir-153 Expression Levels With Gastric Cancer and H. pylori Infection Keywords:

Differentially Expressed Genes (DEGs) , Gastric Cancer , Helicobacter pylori , hsa-mir-196 , hsa-mir-153