Performance Analysis of a Sheath-less Microfluidic Chip With Liquid Electrodes For CountingCells Immersed in a Solution

This paper investigates the performance of amicrofluidic impedance cytometry chip utilizing liquidelectrodes for quantifying suspended cells. The chip design,devoid of the sheath, alleviates fabrication and operationalchallenges. Utilizing the same needles for cell injection andimpedance measurement simplifies the setup. By observingthe simultaneous passage of cells through the channel, it'snoted that multiple cells elevate impedance levels, correlatingwith cell count. The impact of channel dimensions onimpedance measurement is explored, revealing that with asensitivity of 0.1%, individual cells can be detected in a ~180μm diameter channel, while smaller channels risk clogging.High-frequency cell properties are considered, revealing thatat frequencies up to 1 GHz, channel impedance becomes morecapacitive, aiding cell discrimination based on intrinsicproperties

Performance Analysis of a Sheath-less Microfluidic Chip With Liquid Electrodes For CountingCells Immersed in a Solution Keywords:

Liquid electrodes , Microfluidics Impedancecytometry(MIC) , Cell counting , Frequency