Study the effect of internal γ-Aminobutyric acid on physiological and biochemical changes of strawberry (Fragaria × ananassa Duch) under heat stress

The purpose of this study was to investigate the metabolism and role of gamma-aminobutyric acid as an effective compound in inducing stress tolerance in plants, in cultivars of strawberry ʻ Camarosaʼ (heat-sensitive) and ʻKurdistanʼ (heat-tolerant). For this purpose, the strawberry plants were transferred to Isfahan University of Technology on 15 November 2021 and with a fully expanded 5–6 leaf stage, they were transferred to growth chambers with temperatures of 25, 30, 35 and 40 ºC, relative humidity of 70% and 1200 lux of light. After 10 hours of temperature stress, the plants were taken out of the growth chamber and some physiological, biochemical and molecular traits were measured. In order to investigate the expression of the Succinic semialdehyde dehydrogenase gene, sampling was done at 0, 2, 5 and 10 hours after the start of a temperature stress of 40 ºC. This research was conducted as a completely randomized mixed-analysis experiment. The results of the experiment showed that high temperature (40 ºC) in cultivar ʻCamarosaʼ significantly 20.43% decrease the content of gamma-aminobutyric acid and 12.19% the amount of soluble carbohydrates, as well as 43.81% increased the percentage of ion leakage, 20.66% proline, and 200% in injury rating value compared to the control (temperature of 25 ºC). In contrast, High temperature stress (40 ºC) in the cultivar ʻKurdistanʼ caused a 17.78% increase in gamma-aminobutyric acid compared to the control treatment. Injury rating value was statistically non-significant in the cultivar ʻKurdistanʼ at a temperature of 40 ºC compared to the control. The expression level of the SSADH gene (the key gene in the entry of GABA into the tricarboxylic acid pathway) was constant in cultivar ʻCamarosaʼ, but in cultivar ʻKurdistanʼ, the gene expression level increased significantly 2 hours after heat stress.

Study the effect of internal γ-Aminobutyric acid on physiological and biochemical changes of strawberry (Fragaria × ananassa Duch) under heat stress Keywords:

Abiotic stress , Climate change , GABA , Succinic semialdehyde dehydrogenase , تنش غیرزیستی , تغییر اقلیم , سوکسنیک سمی آلدهید دهیدروژناز , گابا