Synthsis, characterization and spectral estimation of Nickel(II), Copper (II) and their Complexes Activity as Antibacterial

The study includes the synthesis and characterization of new azo compound 4-((2-amino-5-nitrophenyl)diazenyl)benzenesulfonic acid as reagent and their complexes with the two metals nickel (II) and copper (II). The complexes were prepared by mixing the azo ligand as a primary ligand and sodium pyrophosphate and malonic acid as secondary ligands using metal-ligand in a 1:1:1 mole ratio. Reagent and complexes were characterized by spectroscopic methods FTIR, UV-Visible, Atomic absorption, C.H.N and GC-Mass. The in-vitro biological activity of the ligand and the metal complexes were screened against the Gram-negative and Gram-positive pathogenic bacteria Serratia marcens and Pseudomonas aeruginosa.The copper complexes showed ahigh efficiency of antimicrobial activity compared with the ligand. Spectroscopic methods were developed to estimate nickel and copper, the best conditions was study which gave high sensitivity. It showed Linear range at 1-10 ppm with a correlation coefficient of (0.9901-0.9924). The detection limit was between (0.0715-0.0895) and the quantitative limit was between (0.2166-0.2713) ppm, the Sandel sensitivity was between (0.0166-0.0208) μg/cm2 and the Recovery% was (100.6177-101.4508).

Synthsis, characterization and spectral estimation of Nickel(II), Copper (II) and their Complexes Activity as Antibacterial Keywords:

Azo ligand , Nickel(II) Complexes , copper(II) complexes , Spectroscopic methods