Bioinformatic Analysis of Changes in TP53 and hsa-miR-122-5p Expression and Their Interaction in Gastric Adenocarcinoma

The most common and lethal type of stomach cancers, which arise from glandular cells of the stomach, is gastric adenocarcinoma. Changes in TP53 and hsa-miR-122-5p expression, and their interactions, were analyzed by using bioinformatics analysis in this study on gastric cancer. The TP53 protein is among the tumor suppressor genes related to the cell cycle and response to DNA damage. Changes in the expression pattern of TP53 may be associated with the onset of cancer. On the other hand, hsa-miR-122-5p is a microRNA involved in the regulation of some gene expressions. Results indicated that TP53 was overexpressed, while has-miR-122-5p was downregulated in the cancer samples. The relation between TP53 and hsa-miR-122-5p may point to the increased expression of TP53 linked with the downregulation of hsa-miR-122-5p. The TargetScan analysis suggests that hsa-miR-122-5p may bind to the 3' UTR region of TP53 and modulate its expression regulation. It is indicated that the interaction of TP53 with hsa-miR-122-5p may be considered a main regulatory mechanism; further experimental studies are needed to confirm these findings for an advanced understanding of its impact on TP53 gene expression. TP53 expression changes and hsa-miR-122-5p were gained from the TCGA data obtained from the UALCAN database in this study. One of the bioinformatics tools that has been used to predict how microRNAs interact with genes is TargetScan Human. Using this tool, we investigated the interaction of hsa-miR-122-5p with the 3' UTR region of the TP53 gene. by performing an appraisal with this tool, it was determined which target regions of the TP53 gene are under the control of hsa-miR-122-5p. Results on the possible effects of this microRNA on TP53 gene expression were investigated. In general, the results indicate that, although miR-122-5p interacts with TP53, such interaction is not very influential in reducing TP53 expressions. The investigation of such interactions might provide further insight into the molecular mechanisms involved in gastric cancer and even into the identification of new biomarkers since the TP53 gene plays a major role in the disease and miRNAs play an important role in the regulation of gene expression. These interactions between TP53 and hsa-miR-122-5p can thus be used as potential diagnostic and prognostic markers of gastric adenocarcinoma. High levels of TP53 expression, combined with low levels of hsa-miR-122-5p, might give critical information on the condition of disease progression, tumor response to therapy, and diagnosis, aiding in the therapeutic approach in improving cancer.

Bioinformatic Analysis of Changes in TP53 and hsa-miR-122-5p Expression and Their Interaction in Gastric Adenocarcinoma Keywords:

TP53 , hsa-mir-122-5p , gastric cancer , gastric adenocarcinoma , bioinformatics