Optimization of the process of cryopreservation of reproductive cells in male pikeperch, Sander lucioperca

The process for cryopreservation of pikeperch sperm was studied as part of the research. The purpose of these studies is to study the effect of different concentrations of DMSO cryoprotectant during cryopreservation of pikeperch sperm on sperm quality indicators. The study of sperm quality was carried out according to generally accepted methods, in which the following indicators were determined: the concentration of fresh sperm, the motility of spermatozoa of fresh and defrosted sperm, and the sperm activity time. In the course of the research, we studied the effect of cryoprotectant concentration on the motility of spermatozoa, in which good results were obtained when using DMSO with a concentration of 10%, which was 68.2±2.24% in the first group and 58.8±2.16% in the second group comparing to DMSO at 20% concentration (p<0.01). The highest motility rate of spermatozoa was observed with sperm freezing with equilibration of 68.2±2.24% in the first group and 58.8±2.16% in the second group compared with equilibration (p<0.01). When studying the sperm activity time in two groups, high results are observed when frozen with DMSO 10% cryoprotectant 883.4±15.12 s and 799.4±10.32s compared to DMSO 20% cryoprotectant, respectively (p<0.05). Of the two groups studied, the group with hormonal stimulation showed the best results in terms of sperm quality. Thus, when studying the protocol for cryopreservation of pikeperch sperm, we found that DMSO cryoprotectant at a concentration of 10% has a positive effect on the quality of deforested sperm.

Optimization of the process of cryopreservation of reproductive cells in male pikeperch, Sander lucioperca Keywords:

Cryopreservation , Cryoprotector , Reproductive cells , Pikeperch , Sperm