Association of adenosine deaminase (ADA) gene 4223A/C polymorphism with obesity in Ardabil, Iran

Objective: Adenosine deaminase (ADA) is a polymorphic enzyme which has an important role in the modulation of insulin bioactivity. The ADA gene polymorphism seems to contribute to the degree of obesity. The aim of this study is to examine the role of ADA gene polymorphism in randomly selected obese subjects from Ardabil Province, Iran. Methods: This case-control study included 170 obese subjects (BMI ≥30) and 200 healthy (BMI ≤30) subjects recruited from Ardabil province's cities. Before the study, these individuals provided approximately 5 ml of blood for molecular tests. We extracted DNA from the blood samples using a standard phenol chloroform procedure. The region that contained the ADA, 4223A/C polymorphism was genotyped by PCR and restriction fragment length polymorphism PCR-RFLP, and the collected data were analyzed using the X2 test. Results: The obese group had significantly increased allele A frequency compared with the control (p=0.034). Conclusion: Our findings suggest a role for the ADA gene in the obesity. Larger studies of other populations are required to confirm these findings.

Association of adenosine deaminase (ADA) gene 4223A/C polymorphism with obesity in Ardabil, Iran Keywords:

Obesity , ADA , Polymorphism , چاقی , آنزیم آدنوزین دآمیناز , چند ریختی