Green synthesis and cytotoxicity effect of silver nanoparticles by using the extract of Tuber spp.

Objective: Recently, green synthesis of silver nanoparticles has been performed out through plants, fungi, bacteria, and algae because this is a simple, low-cost, and environmentally friendly method. This technique can be a suitable alternative to physical and chemical methods. In this study, we assess green synthesis of silver nanoparticles by the truffle fungus) Tuber spp. (extract and MTT assessment of its cytotoxicity. Methods: We used the Tuber spp.Extract as the reducing agent for the biosynthesis of silver nanoparticles. The sizes, structural, optical and morphological properties of the nanoparticles were analyzed by FT-IR, X-ray diffraction and field emission scanning (transmission; TEM) electron microscope. The cytotoxicity effect of silver nanoparticles on cell lines was evaluated by the MTT assay after 24 h. Result: UV-Vis spectrum exhibited an absorption band at around 400-450 nm that suggested the formation of biological silver nanoparticles. The size and morphological properties of nanoparticles were assessed by TEM which showed that the particles had spherical shapes with diameters of approximately 15-30 nm. The MTT assay revealed a dose- and time-dependent anti-proliferative effect of the silver nanoparticles. Conclusion: The extract of Tuber spp.has the ability to reduce theAg+ ion to silver nanoparticles. Silver nanoparticles produced by green synthesis have good anti-proliferative effects compare to the Tuber spp. extract.

Green synthesis and cytotoxicity effect of silver nanoparticles by using the extract of Tuber spp. Keywords:

Green synthesis , Tuber spp , Silver nanoparticle , سنتز سبز , عصاره قارچ دنبلان , نانوذرات نقره