A Review on Cell Sheet Engineering Using Temperature Responsive Culture Dishes for Tissue Engineering Applications

Functional disorder in different tissues is a consequence of cell damage in a part of a tissue that can occur because of diseases, traumas, or accidents. Organ transplantation has so far been the only treatment approach for these damages; however, transplantation therapies have been greatly limited by the serious shortage of donors or immune rejection. One of the alternative approaches is tissue engineering that recently has attracted the tremendous attentions of many researchers. Cell sheet engineering is a technology that can construct bioengineered sheet-like tissues without the need of using scaffolds and it is called “scaffold-free tissue engineering”. Cells are cultured at 37℃ on the surfaces grafted with the temperature responsive polymer “poly (N-isopropylacrylamide)”. Then, the cell sheet is harvested with a simple reduced-temperature treatment up to 20℃ and transplanted directly onto the injured surface without sutures or glues. In the present article, researches and experiments in the field of cell sheet engineering have been paid attention. Moreover, the advantages and challenges of this method have been discussed.

A Review on Cell Sheet Engineering Using Temperature Responsive Culture Dishes for Tissue Engineering Applications Keywords:

Tissue Engineering , Poly (n-isopropylacrylamide) , Temperature responsive polymer , مهندسی لایه سلولی , پلی ان ایزو پروپیل اکریل آمید , پلیمر حساس به دما